Bioreactor and Bioprocess Design Issues in Enzymatic Hydrolysis of Lignocellulosic Biomass

Saccharification of lignocellulosic biomass is a fundamental step in the biorefinery second generation feedstock. The physicochemical and enzymatic processes for depolymerization into simple sugars has been achieved through numerous studies several disciplines. present review discusses development technologies saccharification industrial processes. kinetics cellulolytic enzymes involved polysaccharide hydrolysis discussed as starting point design most promising bioreactor configurations. main process configurations—proposed so far—for have analyzed. Attention was paid to configurations, operating modes possible integrations this operation within biorefinery. focus on minimizing effects product inhibition enzymes, maximizing yields concentration hydrolysate, reducing impact enzyme cost whole process. last part focused an emerging based catalytic action laccase applied lignin alternative consolidated pretreatments. laccases-based oxidative terms characteristics that can affect unit where laccases or laccase-mediator system be used delignification.